RNFtools manual

This is the manual of RNFtools which is an associate software package for Read Naming Format (RNF), a generic format for naming simulated Next-Generation Sequencing reads.

RNFtools can be used for simulation of NGS reads, evaluation of mappers, and conversion of RNF-compliant data.


  1. Břinda, V. Boeva, G. Kucherov. RNF: a general framework to evaluate NGS read mappers. Bioinformatics 32(1), pp. 136-139, 2016. [doi:10.1093/bioinformatics/btv524]

Table of contents

Introduction to RNFtools.
Tutorials demonstrating installation and basic usage of RNFtools.
Automatically generated documentation for RNFtools (including CLI variant rnftools).
Additional information
FAQs, supplementary information about RNFtools, information about state-of-the-art read simulators.